High-throughput Sequencing Panels

GCATbio has proprietary microchip-based Massive in Parallel Synthesis (mMPS) platform, which enables high-throughput, high-fidelity DNA synthesis. Combined with proprietary and optimized probe design tools, we provide a one-stop high-throughput sequencing panel solution to meet your needs. Our custom high-throughput sequencing panel service offers exceptional flexibility, allowing you to introduce specific designs based on the target sequence. Whether you are starting from scratch or adding targets to an existing panel, our solutions can meet requirements for different panel sizes, target regions, and multiplex enrichment.

Key Benefits

Fast Customization And Optimization

End-to-end services from probe design and synthesis to verification, delivering optimal performance with the shortest turnaround time.
Flexible addition of target regions or enhancement of probe density in existing regions without compromising performance.
Custom library preparation kits tailored to downstream applications for seamless workflow integration.

Exceptional And Reliable Performance

Proprietary algorithm and design tool enable specific design of probes based on variant type and gene complexity, achieving. efficient capture of a range of target sizes while minimizing nonspecific capture and redundancy.
Proprietary mMPS platform ensures high-fidelity synthesis of precisely designed probes.
Rigorous quality control and verification processes ensure high capture efficiency, specificity, and consistency.

Dedicated Technical Support

Our team of passionate scientists and technical experts are always available to support your projects.

GCATbio has developed a proprietary DNA probe design tool, ingeniously integrating the advantages of traditional flat design and novel tile design, further enhancing capture efficiency. Simultaneously, they innovatively established a probe evaluation mechanism based on off-target penalty and cross-dimer penalty, enabling the classification of each probe's off-target risk. This allows the probe design platform to automatically identify, avoid, and optimize probes with higher off-target risk, thereby improving on-target rates during the design phase.

Core advantages of probe design platform

Effectative probe design for specific point mutations and fusion genes

Automatic compensation for low capture efficiency of individual probes

More accurate identification of off-target and cross-dimer probes

GCATbio offers a one-stop solution covering panel design, synthesis, validation, and optimization, ultimately delivering complete customized assay kits. Additionally, as ISO 13485 certified, GCATbio also provide CDMO services for NGS IVD products.

How It Works

Order Submission

Confirmation of the target region and capture type

Probe Design

Proprietary probe design platform

Probe design can be completed in as fast as 3 days

Probe Synthesis & QC

120 nt biotin-labeled DNA probes

Rigorous QC (ISO 13485)

NGS Performance Verification

High throughput sequencing platform

Standard and efficient workflow

Verify on-target rate, uniformity

Delivery

Probe synthesis QC report

NGS capture performance verification report

Product shipment

Customized Multi-cancer Detection Panel

GCATbio provides an IVD company with a customized panel service for screening multiple cancers. The Panel size is 346 kb, covering 1460 target sites, including 210 indels and 91 RNA fusion sites. Based on GCATbio's unique probe design tool, a total of 3034 probes were designed. Sequencing results showed excellent probe uniformity, with an overall coverage rate of 99.1% and an on-target rate of 82%.

Capture Performance