High Throughput Gene Synthesis Solution
High Throughput Gene Synthesis Solutions
Gene synthesis presents immense potential across various fields, from biopharma, agriculture and food science to bioenergy. It can be used for drug development, gene therapy, crop improvement, and biofuel production. However, current limitations of mainstream methods in cost, efficiency, accuracy, and synthesis length have hindered the widespread adoption of this technology.
Bringing over a decade of technical expertise, GCATbio's R&D team leveraged extensive experience from the Synthetic Yeast Genome Project (Sc2.0) to develop end-to-end high throughput gene synthesis solutions backed by our proprietary microchip-based massive in parallel (mMPS) DNA synthesis technology, automated gene assembly platforms, and high-throughput sequencing solutions. We are committed to addressing key challenges in gene synthesis workflows and delivering efficient and reliable services to customers while driving technological advances and innovating applications.
Challenges in Gene Synthesis
Gene synthesis begins with the design and chemical synthesis of oligonucleotides. The cost of gene synthesis is directly linked to that of oligo synthesis, which amounts to high expenses for long fragments or genomes. On the other hand, gene length is critical for downstream applications. From small-scale protein optimization to genomic research, the required lengths can range from 10 Kb to Mb. These sequences all need to be assembled from scratch, posing numerous challenges such as low assembly success rates, difficulties in synthesizing long fragments, complex experimental procedures, long turnaround times, and quality control issues. These obstacles severely limit the scalability and widespread use of gene synthesis for large-scale production.
History of DNA Synthesis
Lower Gene Synthesis Costs with mMPS Technology
Cost-effective Capacity
We offer more economical and pragmatic gene synthesis solutions than the conventional nmol-scale column-based approach. While chip-based synthesis is often limited to fmol scales, our technology operates at higher capacities of pmol scale per oligo, allowing direct use in gene synthesis without the need for amplification or unpooling.
Automated Sorting
Each chip is QR-coded, enabling fully automated identification, sorting, and pooling of a massive number of oligos. This facilitates the simultaneous synthesis of oligos for multiple genes.
High Throughput
Up to a million oligos can be synthesized in a single run, allowing for rapid generation of oligos for thousands of genes, suitable for large-scale, highly standardized applications.
GCATbio's mMPS-M200 high throughput chip-based synthesizer went into mass production in 2023
Automated Gene Assembly Platform Enables Efficient Gene Synthesis
After parallel synthesis of multiple oligos on the mMPS synthesizer, we conduct polymerase cycling assembly (PCA)-based assembly and error correction on our automated gene assembly platform, achieving accurate assembly of genes for high-throughput synthesis.
Shortened turnaround time
High-precision, high-throughput liquid handling stations significantly improve efficiency and accuracy, enabling standardized workflows that facilitate timely delivery of thousands of genes within a short timeframe.
Reduced production costs
The automated assembly platform drastically increases throughput and production capacity, further lowering costs to provide high-quality, cost-effective gene synthesis services.
Enhanced success rates for long fragment assembly
High-precision, high-throughput liquid handling stations minimize manual intervention, enabling standardized workflows that greatly improve the success rates of one-step assembly.
High-Throughput Sequencing Verification Ensures Gene Synthesis Quality
Sequencing verification is essential in gene synthesis, as it ensures the accuracy and completeness of synthesized DNA by verifying that the synthesized sequence matches the target design. Through sequencing, potential errors or mutations can be detected and corrected, guaranteeing the quality and function of the synthesized genes. While Sanger sequencing is considered the industry standard for gene synthesis verification, it presents drawbacks of low throughput and high costs. To address these limitations, we have pioneered the use of the MGI G99 platform for sequence verification of synthesized genes, which enables testing of thousands of genes in a single run. Compared to traditional Sanger sequencing, this approach drastically increases throughput while generating larger datasets for in-depth analysis, thereby enhancing the reliability of sequence verification.
Services
Gene Fragment Synthesis
We can synthesize gene fragments up to 1 kb and have them shipped in as little as 2 days. Delivered as lyophilized PCR products, our synthesized DNA saves you time, effort, and cost, while meeting your needs in genetic engineering, gene editing, protein expression and functional studies, antibody drug screening, vaccine research, and more.
Clonal Gene Synthesis
Leveraging our advanced mMPS DNA synthesis, automated gene assembly, and high-throughput sequencing verification technologies, we are dedicated to providing you with fast, efficient, high-quality gene synthesis services. Simply provide us with the desired nucleotide or amino acid sequence, and we will deliver the 100% accurate gene sequence, cloned into any vector of your choice.
Metabolic Pathway and Genome Synthesis
As a leader in DNA synthesis with over a decade of technical expertise and contributions to the global Synthetic Yeast Genome Project (Sc2.0), our exceptional team can provide you with custom metabolic pathway synthesis and genome synthesis/assembly services, delivering more economical, efficient, and standardized solutions to power your life science research.