Life Science Products
Alpha Reverse Transcriptase(200 U/μL)
| Cat. No. | Size | Price (USD) |
|---|---|---|
| LS-EZ-E-00005O | 50 μL | $146.00 |
| LS-EZ-E-00005P | 1 mL | Inquire |
| LS-EZ-E-00005Q | 10 mL | Inquire |
*Consult your local salesman for specific prices
Product Description
Alpha Reverse Transcriptase (RT) is an engineered version of M-MuLV Reverse Transcriptase with reduced RNase H activity. It boasts high sensitivity, excellent processivity, and a pronounced affinity for RNA templates. Notably, it can stably generate cDNA as long as 9 kb.
Applications
Key Benefits
Contents
| Content | LS-EZ-E-00005O | LS-EZ-E-00005P | LS-EZ-E-00005Q | STORAGE CONDITION |
|---|---|---|---|---|
| Alpha Reverse Transcriptase (200 U/μL) | 50 μL | 1 mL | 10 mL | -20 ± 5°C |
| 5× First Strand Buffer | 250 μL | 5 mL | 100 mL | -20 ± 5°C |
| 0.1 M DTT | 120 μL | 1 mLx3 | 10 mLx2 | -20 ± 5°C |
Storage, Shipping, and Shelf Life
Ship on dry ice. Store at -20 ± 5°C. Avoid repeated freeze-thaw cycles. Shelf life: 24 months.
Application Cases
1. RT-qPCR
1) Using the two-step RT-qPCR method, the 1.6 kb housekeeping gene B2M was amplified. The starting template was five 10-fold serial dilutions of HeLa cell total RNA (from 1 pg to 10 ng), two replicates each. Alpha RT stably detects starting templates as low as 10 pg.
2. Standard Bulk RNA-Seq
2) RNA-seq libraries were prepared from Universal Human Reference RNA (UHRR, Invitrogen™) and sequenced on BGISEQ-500 (PE100). Alpha RT showed good performance comparable to competitor T, with relatively consistent Bulk RNA-Seq data.