Life Science Products

RNase Inhibitor(40 U/μL)

Cat. No.	Size	Price (USD)
LS-EZ-E-00006O	50 μL	$35.1
LS-EZ-E-00006P	1 mL	$429.00
LS-EZ-E-00006Q	100 mL	inquire

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Product Description

This product efficiently inhibits RNase A, B, and C activity. It is ineffective against eukaryotic RNase T1, T2, U1, U2, and CL3 or prokaryotic RNase I and H.

Applications

Protection of RNA samples

cDNA synthesis

In vitro transcription or translation

RNA library preparation

Single-cell RNA library preparation

Key Benefits

Potent RNase inhibition

Broad applications: Suitable for reverse transcription, RT-PCR, RT-qPCR, RNA-Seq, and single-cell sequencing

Content	LS-EZ-E-00006O	LS-EZ-E-00006P	LS-EZ-E-00006Q	STORAGE CONDITION
RNase Inhibitor (40 U/μL)	50 μL	1 mL	100 mL	-20 ± 5°C

Storage, Shipping, and Shelf Life

Ship on dry ice. Store at -20 ± 5°C. Avoid repeated freeze-thaw cycles. Shelf life: 24 months.

Application Cases

1. Neo Primaries RNase Inhibitor demonstrates higher RNase inhibition efficiency and performs well in reverse transcription and RNA-Seq assays.

1) RNase Inhibitors from different vendors were mixed with 300 ng of RNA substrate and 100 pg of RNase A, and incubated at 37°C for 1 h; residual RNA was quantified using RNA Qubit HS Assay Kit (9 replicates per group). The control group did not contain RNase Inhibitors. 2) RNA Ladder templates were mixed with RNase Inhibitors from different vendors. First-strand cDNA synthesis was performed using TransFlex I Reverse Transcriptase (Cat. No.: LS-EZ-E-00005), and the products were analyzed with alkaline agarose gel electrophoresis. 3) RNA sequencing library preparation was conducted using RNase Inhibitors from different vendors, and the libraries were quantified and analyzed on agarose gels.

2. scRNA-Seq in Single Cells

Neo Primaries RNase Inhibitor/Reverse Transcriptase captures more genes per cell and yields better sequencing quality.