Life Science Products

dsDNA HS Assay Kit

Cat. No.	Size	Price (USD)
LS-DY-R-00001	100 assays	Inquire
LS-DY-R-00002	500 assays	Inquire

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Product Description

dsDNA HS Assay Kit is intended for fluorescent dye-based quantification of double-stranded DNA (dsDNA). It contains a concentrated assay reagent, dilution buffer, and prediluted DNA standards. The kit is highly selective for dsDNA and demonstrates good linearity within the 0.1–120 ng range, allowing for fast, accurate, sensitive, and specific quantification of dsDNA. This kit is compatible with Qubit Fluorometer 1.0, 2.0, 3.0, and 4.0 as well as microplate readers.

Applications

Precise quantification of dsDNA

Key Benefits

High sensitivity： the assay kit is designed to be accurate for initial dsDNA sample concentrations of 0.005 to 120 ng/μL, providing a detection range of 0.1−120 ng.

High specificity: Highly specific to dsDNA and tolerant against contaminants

Fast-binding dye: Requires only 2-minute assay incubation

High stability: Rigorously controlled production ensures lot-to-lot stability

Content	Component	LS-DY-R-00001	LS-DY-R-00002	STORAGE CONDITION
dsDNA HS Reagent	200× DMSO Solution	250 µL	1.25 mL	2–8°C, protected from light
dsDNA HS Buffer	N/A	50 mL	250 mL	≤30℃
Standard # 1	0 ng/µL dsDNA Solution	1 mL	5 mL	2-8℃
Standard # 2	10 ng/µL dsDNA Solution	1 mL	5 mL	2–8°C, or -20°C for long-term storage

Storage, Shipping, and Shelf Life

Ship with ice packs.2–8°C, protected from light, or -20°C for long-term storage. Shelf life: 1 year.

Notes

• Fluorescent dyes are susceptible to photobleaching. Protect from light to minimize photobleaching. • Equilibrate to room temperature and mix well by inverting at least 10 times before use. • Before each use, mix each DNA standard well by inverting and pulse spin for a few seconds (do not vortex). • For your safety, please wear personal protective equipment when using the product. • This product is for research use only.

Application Cases

a）High specificity

Three samples (DNA, RNA, and 1:1 DNA/RNA mix) were assayed within the linear range (0.1–120 ng) using Neo Primaries' and Vendor T's kits. Fluorescence measurements were plotted against sample concentrations (see figure above). The Neo Primaries kit is capable of accurate quantification of dsDNA in the presence of RNA, with comparable performance to Vendor T's product.

b）Broad linear range

Neo Primaries' and Vendor T's kits were used to assay dsDNA samples of varying concentrations. Fluorescence was measured with a Qubit fluorometer. Results indicate good linearity for dsDNA samples within the 0.1–120 ng range.