Life Science Products
T4 Polynucleotide Kinase(10 U/μL)
| Cat. No. | Size | Price (USD) |
|---|---|---|
| LS-EZ-E-00015O | 200 μL | Inquire |
| LS-EZ-E-00015P | 4 mL | Inquire |
| LS-EZ-E-00015Q | 10 mL | Inquire |
*Consult your local salesman for specific prices
Product Description
T4 Polynucleotide Kinase (T4 PNK) is a polynucleotide 5'-hydroxyl-kinase that catalyzes the transfer of a γ-phosphate from ATP to the 5' hydroxyl terminus of single- or double-stranded DNA and RNA, oligonucleotides, or mononucleotides bearing 3'-phosphates. It is widely applicable to 5' phosphorylation of oligonucleotides, DNA, or RNA, as well as DNA or RNA end labeling.
Applications
Key Benefits
Product Parameter
| Molecular Weight | 48 kDa |
| Concentration¹ | 10 U/μL |
| Storage Buffer | 10 mM Tris-HCl, 50 mM KCl, 0.1 µM ATP, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.4 @ 25°C |
| Storage Condition | Store at -20 °C. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 24 months |
| Shipping Condition | Ship on dry ice |
¹Unit definition: One activity unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of γ-phosphate from ATP to a 5'-OH terminus of DNA in 30 min at 37°C.
Contents
| Content | LS-EZ-E-00015O | LS-EZ-E-00015P | LS-EZ-E-00015Q | STORAGE CONDITION |
|---|---|---|---|---|
| T4 PNK | 200 μL×1 vial | 4 mL×1 vial | 10 mL×1 vial | -20°C |
| 10× PNK Reaction Buffer | 1 mL×1 vial | 10 mL ×2 vial | 50 mL×1 vial | -20°C |
Storage, Shipping, and Shelf Life
Ship on dry ice. Store at -20°C; avoid repeated freeze-thaw cycles. Shelf life: 24 months.
Application Cases
1.HTS library preparation
YH gDNA was fragmented and double-size-selected, followed by end repair, dA-tailing, adapter ligation, and PCR enrichment. End repair was performed using Neoprimaries T4 PNK or a competitor enzyme; the other materials were the same. The adapter ligated and PCR enriched products were quantified, and the libraries were sequenced on Sequencing Platform. The results demonstrate comparable performance for Neoprimaries T4 PNK and the competitor.
2.library preparation
PCR-free PE100 libraries were prepared from 50 ng of fragmented YH gDNA using Neoprimaries T4 PNK or a competitor enzyme, and sequenced on Sequencing Platform. The results show that Neoprimaries T4 PNK has comparable performance to the competitor and is suitable for library preparation.